ABSTRACT
Objective To study the effect ofRunfei Zhisou Pills (RZP) on cell apoptosis in rat model of chronic obstructive pulmonary disease (COPD) and its mechanism.Methods Totally 32 male Wistar rats were randomly divided into control group,model group,1-month RZP group,and 3-month RZP group.Rats in 1-month and 3-month RZP group,received RZP of 0.9 g/kg once daily for 1 and 3 months respectively,and rats in control and model groups received water with equal volume for 1 month.The rat COPD model was established using tobacco smoke combined with intratracheal instillation of LPS.Morphological changes of lung tissue in COPD rats were observed with HE staining under light microscope.The VEGF levels in bronchoalveolar lavage fluid (BALF) were detected by ELISA.The VEGF and VEGFR2 protein expression in lung tissues was measured by Western blotting.Cell apoptosis in lung tissues was detected with TUNEL method,and apoptosis index (AI) was counted.Results Compared with control group,the pathological changes of lung tissue in model group were obvious,the AI significantly increased,VEGF in BALF and VEGF and VEGFR2 in lung tissue decreased significantly.Compared with model group,morphological improvement of lung tissue was obvious,the AI significantly decreased,and VEGF in BALF,VEGF and VEGFR2 in lung tissue increased significantly in 1-month and 3-month RZP groups.Conclusion RZP can inhibit cell apoptosis of COPD,and the mechanism may be related with up-regulation of VEGF and VEGFR2.
ABSTRACT
Objective To study the diagnostic significance of the gray degree of lip-around and facial edge skin on the spleen-stomach symptoms.Methods With the International ColorCheck,160 cases of facial digital images were got from physical examination groups.The gray degree of the upper and lower lip-around and facial edge skin were judged.Questionnaires of spleen-stomach symptoms were also collected from the people.Results Logistic results showed that the dark upper lip-around skin were closely related with dry mouth,stomach pain,etc; the dark lower lip-around skin were closely related with hiccups,etc; the dark facial edge skin were closely related with bloating- afternoon,bad-breath,vomiting,cool-stomach,the thick white fur and so on.Conclusion The gray degree of lip-around and facial edge skin implied the status of the spleen and stomach function,which could be used as diagnostic indicators for spleen and stomach symptoms.